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rabbit anti-human cd3 antibody clone sp7  (Thermo Fisher)


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    Structured Review

    Thermo Fisher rabbit anti-human cd3 antibody clone sp7
    Rabbit Anti Human Cd3 Antibody Clone Sp7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-human+cd3+antibody+clone+sp7/antibody+against+cd3+rabbit+monoclonal+sp7/pm39244214-111-4-14
    Average 90 stars, based on 1 article reviews
    rabbit anti-human cd3 antibody clone sp7 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers.
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti- human CD3 antibody (clone SP7—Epredia RM- 9107- S Thermo Fisher Scientific) was used.

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti-human CD3 antibody (clone SP7—Epredia RM-9107-S Thermo Fisher Scientific) was used.

    Activation Assay:

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers.
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti- human CD3 antibody (clone SP7—Epredia RM- 9107- S Thermo Fisher Scientific) was used.

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti-human CD3 antibody (clone SP7—Epredia RM-9107-S Thermo Fisher Scientific) was used.

    Amplification:

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers.
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti- human CD3 antibody (clone SP7—Epredia RM- 9107- S Thermo Fisher Scientific) was used.

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti-human CD3 antibody (clone SP7—Epredia RM-9107-S Thermo Fisher Scientific) was used.

    Expressing:

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers.
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti- human CD3 antibody (clone SP7—Epredia RM- 9107- S Thermo Fisher Scientific) was used.

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti-human CD3 antibody (clone SP7—Epredia RM-9107-S Thermo Fisher Scientific) was used.

    Control:

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers.
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti- human CD3 antibody (clone SP7—Epredia RM- 9107- S Thermo Fisher Scientific) was used.

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti-human CD3 antibody (clone SP7—Epredia RM-9107-S Thermo Fisher Scientific) was used.

    Fluorescence:

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers.
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti- human CD3 antibody (clone SP7—Epredia RM- 9107- S Thermo Fisher Scientific) was used.

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti-human CD3 antibody (clone SP7—Epredia RM-9107-S Thermo Fisher Scientific) was used.

    Immunopeptidomics:

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers.
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti- human CD3 antibody (clone SP7—Epredia RM- 9107- S Thermo Fisher Scientific) was used.

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti-human CD3 antibody (clone SP7—Epredia RM-9107-S Thermo Fisher Scientific) was used.

    Plasmid Preparation:

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers.
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti- human CD3 antibody (clone SP7—Epredia RM- 9107- S Thermo Fisher Scientific) was used.

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti-human CD3 antibody (clone SP7—Epredia RM-9107-S Thermo Fisher Scientific) was used.

    Sequencing:

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers.
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti- human CD3 antibody (clone SP7—Epredia RM- 9107- S Thermo Fisher Scientific) was used.

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti-human CD3 antibody (clone SP7—Epredia RM-9107-S Thermo Fisher Scientific) was used.

    Clinical Proteomics:

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers.
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti- human CD3 antibody (clone SP7—Epredia RM- 9107- S Thermo Fisher Scientific) was used.

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti-human CD3 antibody (clone SP7—Epredia RM-9107-S Thermo Fisher Scientific) was used.

    Flow Cytometry:

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers.
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti- human CD3 antibody (clone SP7—Epredia RM- 9107- S Thermo Fisher Scientific) was used.

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti-human CD3 antibody (clone SP7—Epredia RM-9107-S Thermo Fisher Scientific) was used.

    MANN-WHITNEY:

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers.
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti- human CD3 antibody (clone SP7—Epredia RM- 9107- S Thermo Fisher Scientific) was used.

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti-human CD3 antibody (clone SP7—Epredia RM-9107-S Thermo Fisher Scientific) was used.

    Activity Assay:

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers.
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti- human CD3 antibody (clone SP7—Epredia RM- 9107- S Thermo Fisher Scientific) was used.

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti-human CD3 antibody (clone SP7—Epredia RM-9107-S Thermo Fisher Scientific) was used.

    Reverse Transcription:

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers.
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti- human CD3 antibody (clone SP7—Epredia RM- 9107- S Thermo Fisher Scientific) was used.

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti-human CD3 antibody (clone SP7—Epredia RM-9107-S Thermo Fisher Scientific) was used.

    Real-time Polymerase Chain Reaction:

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers.
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti- human CD3 antibody (clone SP7—Epredia RM- 9107- S Thermo Fisher Scientific) was used.

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti-human CD3 antibody (clone SP7—Epredia RM-9107-S Thermo Fisher Scientific) was used.

    Immunofluorescence:

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers.
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti- human CD3 antibody (clone SP7—Epredia RM- 9107- S Thermo Fisher Scientific) was used.

    Article Title: MHC class I and II-deficient humanized mice are suitable tools to test the long-term antitumor efficacy of immune checkpoint inhibitors and T-cell engagers
    Article Snippet: For CD3 immunohistochemistry, a rabbit anti-human CD3 antibody (clone SP7—Epredia RM-9107-S Thermo Fisher Scientific) was used.



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    CD56 + cells are not detectable in granulomatosis with polyangiitis (GPA) but are present in granulomas of classical granulomatous diseases. Patient material was derived from and processed in the Heidelberg tissue bank for inflammatory diseases. Sequential paraffin-embedded sections were stained with hematoxylin and eosin (HE) ( left column ), <t>anti-CD3</t> antibody ( middle column ), and anti-CD56 antibody ( right column ). a Normal tonsil tissue served as a positive control for <t>CD3</t> and CD56 staining ( top row ). Representative tissue sections of granulomas from tuberculosis (Tb) (n = 5; second row ), sarcoidosis (n = 5; third row ), and GPA (n = 13; bottom row ) patients. Healthy lung tissue at the border of GPA granulomas contained scattered CD56 + cells (not shown). Arrowheads point to giant cells. N necrosis. Measurement bars indicate 100µm. b Enlarged cutout from the sequential Tb sections shown in (a). Upper arrows , CD3 − CD56 + natural killer (NK) cells; lower arrows , CD3 + CD56 + T cells
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    Image Search Results


    CD56 + cells are not detectable in granulomatosis with polyangiitis (GPA) but are present in granulomas of classical granulomatous diseases. Patient material was derived from and processed in the Heidelberg tissue bank for inflammatory diseases. Sequential paraffin-embedded sections were stained with hematoxylin and eosin (HE) ( left column ), anti-CD3 antibody ( middle column ), and anti-CD56 antibody ( right column ). a Normal tonsil tissue served as a positive control for CD3 and CD56 staining ( top row ). Representative tissue sections of granulomas from tuberculosis (Tb) (n = 5; second row ), sarcoidosis (n = 5; third row ), and GPA (n = 13; bottom row ) patients. Healthy lung tissue at the border of GPA granulomas contained scattered CD56 + cells (not shown). Arrowheads point to giant cells. N necrosis. Measurement bars indicate 100µm. b Enlarged cutout from the sequential Tb sections shown in (a). Upper arrows , CD3 − CD56 + natural killer (NK) cells; lower arrows , CD3 + CD56 + T cells

    Journal: Arthritis Research & Therapy

    Article Title: Peripheral blood natural killer cell percentages in granulomatosis with polyangiitis correlate with disease inactivity and stage

    doi: 10.1186/s13075-015-0851-7

    Figure Lengend Snippet: CD56 + cells are not detectable in granulomatosis with polyangiitis (GPA) but are present in granulomas of classical granulomatous diseases. Patient material was derived from and processed in the Heidelberg tissue bank for inflammatory diseases. Sequential paraffin-embedded sections were stained with hematoxylin and eosin (HE) ( left column ), anti-CD3 antibody ( middle column ), and anti-CD56 antibody ( right column ). a Normal tonsil tissue served as a positive control for CD3 and CD56 staining ( top row ). Representative tissue sections of granulomas from tuberculosis (Tb) (n = 5; second row ), sarcoidosis (n = 5; third row ), and GPA (n = 13; bottom row ) patients. Healthy lung tissue at the border of GPA granulomas contained scattered CD56 + cells (not shown). Arrowheads point to giant cells. N necrosis. Measurement bars indicate 100µm. b Enlarged cutout from the sequential Tb sections shown in (a). Upper arrows , CD3 − CD56 + natural killer (NK) cells; lower arrows , CD3 + CD56 + T cells

    Article Snippet: The following primary antibodies were used: rabbit anti-human monoclonal antibody (mAb) CD3 (clone SP7, immunoglobulin G (IgG), 1:200; Thermo Fisher Scientific, Fremont, CA, USA) and mouse anti-human CD56 mAb (clone 1B6, IgG1, 1:10; Leica Biosystems, Newcastle upon Tyne, UK).

    Techniques: Derivative Assay, Staining, Positive Control

    Natural killer (NK) cell proportions negatively correlate with granulomatosis with polyangiitis (GPA) activity and are increased in inactive GPA. Peripheral blood mononuclear cells from patients and healthy controls (HCs) were isolated using Ficoll-Paque density gradient medium and analyzed by flow cytometry. a Gating strategy. Peripheral blood lymphocytes (PBLs) were determined using forward and sideward scatter (FSC and SSC, respectively). Cell viability was confirmed by negative 7-aminoactinomycin (7-AAD) staining in each experiment. NK cells were determined by costaining of CD3 (negative) and CD56 (positive). b Scatterplot showing the relationship between NK cell proportions and Birmingham Vasculitis Activity Score (BVAS). Note that in one case, two patients are represented by a single dot; both had a BVAS of 3 and NK cell proportions of 15.6 % and 15.8 %, respectively (n = 28). Spearman’s correlation coefficient ( r ) and statistical significance were as indicated on the figure. c and d Box-and-whisker plots. Whiskers represent 10th–90th percentile range, statistical significance is as indicated. Left graph in ( c ) depicts NK cell proportions of PBLs from HCs (n = 29), patients with active GPA (n = 11), patients with inactive GPA (n = 17), and patients with inactive systemic inflammatory control diseases (CD; n = 12). Right graph in ( c ) illustrates NK cell counts in PBLs from patients with active GPA (n = 6), patients with inactive GPA (n = 5), and patients with inactive CD (n = 12). Statistical significance was determined by Kruskal-Wallis test ( p = 0.0002 for relative and p = 0.014 for absolute NK cell numbers) and Dunn’s multiple comparison posttests (asterisks) as shown in the graphs. d T-cell proportions of patients with active (n = 11), inactive (n = 17) GPA and of HCs. Statistical analysis using the Kruskal-Wallis test revealed that CD56 − but not CD56 + T-cell proportions were significantly different ( p = 0.04). Dunn’s multiple comparison posttests were positive where indicated by asterisks. e Receiver operating characteristic curve showing sensitivity and specificity of different NK cell proportions to distinguish inactive from active GPA. NK cells >18.5 % of PBLs determined GPA inactivity with a specificity of 100 % and a sensitivity of 71 %. AUC area under the curve

    Journal: Arthritis Research & Therapy

    Article Title: Peripheral blood natural killer cell percentages in granulomatosis with polyangiitis correlate with disease inactivity and stage

    doi: 10.1186/s13075-015-0851-7

    Figure Lengend Snippet: Natural killer (NK) cell proportions negatively correlate with granulomatosis with polyangiitis (GPA) activity and are increased in inactive GPA. Peripheral blood mononuclear cells from patients and healthy controls (HCs) were isolated using Ficoll-Paque density gradient medium and analyzed by flow cytometry. a Gating strategy. Peripheral blood lymphocytes (PBLs) were determined using forward and sideward scatter (FSC and SSC, respectively). Cell viability was confirmed by negative 7-aminoactinomycin (7-AAD) staining in each experiment. NK cells were determined by costaining of CD3 (negative) and CD56 (positive). b Scatterplot showing the relationship between NK cell proportions and Birmingham Vasculitis Activity Score (BVAS). Note that in one case, two patients are represented by a single dot; both had a BVAS of 3 and NK cell proportions of 15.6 % and 15.8 %, respectively (n = 28). Spearman’s correlation coefficient ( r ) and statistical significance were as indicated on the figure. c and d Box-and-whisker plots. Whiskers represent 10th–90th percentile range, statistical significance is as indicated. Left graph in ( c ) depicts NK cell proportions of PBLs from HCs (n = 29), patients with active GPA (n = 11), patients with inactive GPA (n = 17), and patients with inactive systemic inflammatory control diseases (CD; n = 12). Right graph in ( c ) illustrates NK cell counts in PBLs from patients with active GPA (n = 6), patients with inactive GPA (n = 5), and patients with inactive CD (n = 12). Statistical significance was determined by Kruskal-Wallis test ( p = 0.0002 for relative and p = 0.014 for absolute NK cell numbers) and Dunn’s multiple comparison posttests (asterisks) as shown in the graphs. d T-cell proportions of patients with active (n = 11), inactive (n = 17) GPA and of HCs. Statistical analysis using the Kruskal-Wallis test revealed that CD56 − but not CD56 + T-cell proportions were significantly different ( p = 0.04). Dunn’s multiple comparison posttests were positive where indicated by asterisks. e Receiver operating characteristic curve showing sensitivity and specificity of different NK cell proportions to distinguish inactive from active GPA. NK cells >18.5 % of PBLs determined GPA inactivity with a specificity of 100 % and a sensitivity of 71 %. AUC area under the curve

    Article Snippet: The following primary antibodies were used: rabbit anti-human monoclonal antibody (mAb) CD3 (clone SP7, immunoglobulin G (IgG), 1:200; Thermo Fisher Scientific, Fremont, CA, USA) and mouse anti-human CD56 mAb (clone 1B6, IgG1, 1:10; Leica Biosystems, Newcastle upon Tyne, UK).

    Techniques: Activity Assay, Isolation, Flow Cytometry, Staining, Whisker Assay, Control, Comparison

    Percentages of T-cell subsets in the spleen, thymus, and peripheral blood of HSC-transplanted mice

    Journal: Experimental Animals

    Article Title: NOD- Rag2 null IL-2Rγ null Mice: An Alternative to NOG Mice for Generation of Humanized Mice

    doi: 10.1538/expanim.63.321

    Figure Lengend Snippet: Percentages of T-cell subsets in the spleen, thymus, and peripheral blood of HSC-transplanted mice

    Article Snippet: To identify human cells in the spleen of transplanted mice, the spleens were removed from the mice after blood removal, and tissues were fixed with 10 nM formalin (10 nM Mildform; Wako, Tokyo, Japan), embedded in paraffin, and stained with hematoxylin and eosin (HE), mouse anti-human CD45 antigen monoclonal antibody (clone 2B11+PD7/26; Dako Cytomation, Glostrup, Denmark), rabbit anti-human CD3 monoclonal antibody (clone SP7; Nichirei, Tokyo, Japan), and mouse anti-human CD79a monoclonal antibody (clone JCB117; Nichirei).

    Techniques: Injection